Science University Research Symposium (SURS)
Publication Date
2024
College
Pharmacy, College of
SURS Faculty Advisor
Dr. Amy Ham
Presentation Type
Poster Presentation
Abstract
Proteomics, the study of complex protein mixtures in which liquid chromatography-mass spectrometry (LC-MS) is used to analyze peptide sequences, is commonly utilized to study the double membrane, cell-derived vesicles known as exosomes. Exosomes are double membrane, cell-derived vesicles that contain cell components such as DNA, lipids, and proteins. They function to transport proteins and cellular materials between cells and are involved in the progression and metastasis of cancer. The impact of these extracellular vesicles on cancer spread can be analyzed using proteomics, through the purification of the proteins isolated from the vesicles. The purpose of our research is to analyze protein contents of exosomes for the comparison of the effectiveness of new purification techniques. Using purified exosomes from cell culture media of colorectal cancer cells of the DiFi cell line, we analyzed the proteomic differences between samples with and without the addition of electrophoretic purification. This analysis served to investigate the effectiveness of electrophoresis in 300kD dialysis tubing to separate exosomes from other vesicles and nanoparticles in the cell culture media. The samples were analyzed by LC-MS analysis, and the proteins were identified using database searching through bioinformatics analysis. Identified proteins from samples collected before and after electrophoresis were compared to understand how this technique affects the purification and isolation of exosomes. This process of isolating exosomes can be useful in cancer research by understanding the exchange of information between cells, monitoring cancer progression and developing new drug delivery therapies.
Recommended Citation
Boone, Renee and Osthoff, Cecilia, "The complex protein analysis of purified extracellular vesicles in the study of colorectal cancer" (2024). Science University Research Symposium (SURS). 155.
https://repository.belmont.edu/surs/155
Included in
Amino Acids, Peptides, and Proteins Commons, Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Biological Factors Commons, Complex Mixtures Commons, Medical Biochemistry Commons, Medical Cell Biology Commons, Neoplasms Commons, Oncology Commons, Other Pharmacy and Pharmaceutical Sciences Commons
Comments
The College of Pharmacy has updates its name to the College of Pharmacy and Health Sciences