Belmont University Research Symposium (BURS)

Publication Date

Spring 2024

College

Sciences and Mathematics, College of

Department

Biology, Department of

BURS Faculty Advisor

Rebecca Adams

Presentation Type

Poster Presentation

Abstract

In eukaryotic cells, such as the budding yeast Saccharomyces cerevisiae, mRNA export is the essential process in which mature mRNA is transported from its site of production, the nucleus, through the nuclear pore complex (NPC) to the cytoplasm, where it can then be translated into protein. This is accomplished when a transcript interacts with the mRNA export protein Mex67, which shuttles the message across the NPC. When the cell is under conditions of stress, such as heat shock, most mRNA export is prohibited to allow for selective gene expression for cell recovery. However, some transcripts are able to be selectively exported and expressed in these conditions. An example is SSA4, which encodes a protein that assists denatured proteins to refold under stress conditions and is up-regulated in response to heat shock. It is not known how SSA4 is selectively exported from the nucleus. I hypothesize that an unknown adaptor protein binds to the SSA4 transcript to recruit Mex67. To test this hypothesis, we performed a yeast three-hybrid (Y3H) test by constructing a plasmid that contains the SSA4 ORF and 3’ UTR, in addition to MS2 hairpins. This construct was assessed for interaction with Mex67. No interaction was observed, so we are trying to reproduce these results. If no interaction is observed for an additional time, then we will perform a screen for other proteins that are able to interact with the SSA4 transcript.

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