Testing Whether Transient Binding of Dpb5 Enables Selective Removal of Mex67 During mRNA Export

Publication Date

2026

Presentation Length

Poster/Gallery presentation

College

College of Sciences & Mathematics

Department

Biology, Department of

Student Level

Undergraduate

Faculty Mentor

Rebecca Adams, PhD

Presentation Type

Poster

Summary

The process of mRNA processing in yeast is accompanied by Mex67 as it travels through the nucleus to the cytoplasm. Mex67 is then selectively removed from the mRNA by Dbp5 at the nuclear pore complex to then be used again for the next mRNA. The process in which Dbp5 can selectively remove Mex67 from the mRNA cannot be fully observed. As a result, Dbp5 is assumed to transiently bind to Mex67 which is how it can selectively remove it. To observe this interaction, Gibson assembly was used to create plasmids containing our selected proteins along with URA3 to be then used for Split Ubiquitin Yeast 2 Hybrid. If transient binding occurs between dpb5 and mex67, this will create ubiquitin which will then degrade the ura3, allowing for yeast growth on FOA plates. Our results show that transient binding occurred between Mex67 and Dpb5 as yeast colonies are present on FOA plates.

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