This study used gene expression analysis to explore the specific response to amodiaquine in HCT116 colorectal cancer cells. Real-time PCR revealed that 20 human stress and toxicity genes were upregulated by at least 3 fold in HCT116 colorectal cancer cells treated with AMQ. Of these 20 upregulated genes, 9 were found to be pro-apoptotic and 2 were DNA damage repair genes, which supports our original hypothesis that amodiaquine is inducing a stress response in these cells. These results are consistent with previous research done by Qiao et al. (2013), as DNA damage repair genes were among the genes upregulated in AMQ-treated melanoma cells. While our data show that the majority of upregulated genes were pro-apoptotic and only two 14 were DNA damage repair genes, DNA damage often triggers apoptosis, which suggests that apoptosis is of significant interest regarding the effect of AMQ exposure to HCT116 cells.
Dr. Chris Barton
Pyles, Holly, "Effect of Amodiaquine on Gene Expression and Apoptosis in Colorectal Cancer Cells" (2020). Honors Theses. 6.